WebUsing Southern blot analysis, we further demonstrated that the viral RNAs were predominantly packaged in CsCl purified mature capsids (containing viral genome) than … Web13 feb. 2009 · RNA gel blot analysis of GUS mRNA abundance in leaves of transgenic tobacco plants containing the −935 FaChit1 5′ upstream region fused to the GUS reporter gene after 24 h of different stress treatments: a 100 μg/ml fungal elicitor, b 300 mM mannitol, c 1 mg/ml ethylene, and d mechanical wounding. ddH 2 O was used as mock treatment.
タンパク質のウェスタンブロッティングの基礎
WebATP-induced inward currents were measured in mock-, wild-type or mutant (C90S)-UCH L1-transfected PC12 cells under the conventional whole-cell patch clamp configuration. The amplitude of ATP-induced currents was significantly greater in both wild-type and C90S UCH L1-transfected cells, suggesting that hydrolase activity was not involved but … Web15 aug. 2008 · For northern blotting, dsRNA, DNA or dsRNA plus DNA were transfected into S2 * cells using a standard calcium phosphate method. dsRNA and DNA were prepared in 2× BBS [BES-buffered saline; 50 mmoll –1N,N -bis (2-hydroxyethyl)2-aminoethane-sulfonic acid (Sigma), 0.28 moll –1 NaCl, 1.5 mmoll –1 Na 2 HPO 4, at pH 6.95], followed … fancy beef cuts
生物实验技术---RNA dot bloting 实验笔记 - 哔哩哔哩
Web14 mrt. 2024 · Measurement of RNA stability 20 million LCL and Akata cells were cultured and reactivated using TPA and Butyric acid or mock reactivated using PBS. After 12 and 24 h post reactivation 1 µM Actinomycin D (Sigma … Web6 jun. 2024 · Northern blot检测显示RNase R (+)组中无线性GAPDH的条带，CDR1as和hsa-circRNA 2/3/16能检测到条带，表明线性RNA被消化，而circRNA耐受消化（Memczak S et al., 2013） Northern blot实验使用探针特异性检测内源的circRNA和线性RNA，过程中无PCR扩增，能够真实地反映circRNA和线性RNA的定性定量分析结果，是circRNA鉴定中 … Web27 apr. 2011 · The RNA quality was checked using Bioanalyzer 2100 (Agilent Technologies) before denaturation and electrophoresis (10 μg per lane) on a 10% acrylamide:bis-acrylamide (37.5:1) denaturing gel in 0.5 × TBE buffer in the presence of 8 M urea. The RNA was then transferred onto BrightStar Plus (Ambion) nylon membranes and UV … fancy beef stew